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Recombinant human CYTH2 protein

CAT: 0112-ATGP1752-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1752-500Size:500 µg
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Description
CYTH2, also known as Cytohesin-2 and ARNO, is an ARF-1 guanine nucleotide exchange factor (GEF) . ARF (ADP ribosylation factor) proteins, a group within the RAS superfamily, are GTP-binding proteins central to the process of vesicle budding. This protein promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Also it promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. Recombinant human CYTH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Cytohesin 2, ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
48.9 kDa (422aa) confirmed by MALDI-TOF
Additionnal Information
CYTH2, Cytohesin 2, ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like, ATGP1752-10 µg, ATGP1752-20 µg, ATGP1752-50 µg, ATGP1752-100 µg, ATGP1752-250 µg, ATGP1752-500 µg, ATGP1752-1 mg, ATGP1752-10, ATGP1752-20, ATGP1752-50, ATGP1752-100, ATGP1752-250, ATGP1752-500, ATGP1752-1
References & Citations
Hofmann I., et al. (2007) Curr. Biol. 17:711-716; ; Chardin, P., et al. (1996) Nature 384: 481-484.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol,1mM DTT
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_004219
Uniprot Accession Number
Q99418
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEDGVYE PPDLTPEERM ELENIRRRKQ ELLVEIQRLR EELSEAMSEV EGLEANEGSK TLQRNRKMAM GRKKFNMDPK KGIQFLVENE LLQNTPEEIA RFLYKGEGLN KTAIGDYLGE REELNLAVLH AFVDLHEFTD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF AQRYCLCNPG VFQSTDTCYV LSFAVIMLNT SLHNPNVRDK PGLERFVAMN RGINEGGDLP EELLRNLYDS IRNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVDDPR KPNCFELYIP NNKGQLIKAC KTEADGRVVE GNHMVYRISA PTQEEKDEWI KSIQAAVSVD PFYEMLAARK KRISVKKKQE QP

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