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Recombinant human ECSIT protein

CAT: 0112-ATGP1745-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1745-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF) -6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Evolutionarily conserved signaling intermediate in Toll pathway mitochondrial, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
24.6 kDa (222aa) confirmed by MALDI-TOF
Additionnal Information
ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway mitochondrial, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC, ATGP1745-10 µg, ATGP1745-20 µg, ATGP1745-50 µg, ATGP1745-100 µg, ATGP1745-250 µg, ATGP1745-500 µg, ATGP1745-1 mg, ATGP1745-10, ATGP1745-20, ATGP1745-50, ATGP1745-100, ATGP1745-250, ATGP1745-500, ATGP1745-1
References & Citations
Moustakas A., et al. (2003) Genes Dev17:2855-2859; ; Vogel R O., et al. (2007) Genes Dev. 21: 615-624.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_001135937
Uniprot Accession Number
Q9BQ95
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS

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