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Recombinant human ADPRH protein

CAT: 0112-ATGP1633-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1633-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ADPRH (ADP-ribosylarginine hydrolase), also known as ADP-ribose-L-arginine cleaving enzyme, belongs to the ADP-ribosylglycohydrolase family. This protein catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent. Recombinant human ADPRH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Protein ADP-ribosylarginine hydrolase, ARH1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
42.1 kDa (381aa) confirmed by MALDI-TOF
Additionnal Information
ADPRH, Protein ADP-ribosylarginine hydrolase, ARH1, ATGP1633-10 µg, ATGP1633-20 µg, ATGP1633-50 µg, ATGP1633-100 µg, ATGP1633-250 µg, ATGP1633-500 µg, ATGP1633-1 mg, ATGP1633-10, ATGP1633-20, ATGP1633-50, ATGP1633-100, ATGP1633-250, ATGP1633-500, ATGP1633-1
References & Citations
Weber F, et al. (2005) J Clin Endocrinol Metab. 90 (2) :1149-55.; ; Kernstock S, et al. (2009) Acta Crystallogr Sect F Struct Biol Cryst Commun. 65 (Pt 5) :529-32.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001116
Uniprot Accession Number
P54922
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEKYVA AMVLSAAGDA LGYYNGKWEF LQDGEKIHRQ LAQLGGLDAL DVGRWRVSDD TVMHLATAEA LVEAGKAPKL TQLYYLLAKH YQDCMEDMDG RAPGGASVHN AMQLKPGKPN GWRIPFNSHE GGCGAAMRAM CIGLRFPHHS QLDTLIQVSI ESGRMTHHHP TGYLGALASA LFTAYAVNSR PPLQWGKGLM ELLPEAKKYI VQSGYFVEEN LQHWSYFQTK WENYLKLRGI LDGESAPTFP ESFGVKERDQ FYTSLSYSGW GGSSGHDAPM IAYDAVLAAG DSWKELAHRA FFHGGDSDST AAIAGCWWGV MYGFKGVSPS NYEKLEYRNR LEETARALYS LGSKEDTVIS L

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