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Recombinant human GTF2E2 protein

CAT: 0112-ATGP1547-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1547-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and two of 34, 000 (TFIIE-beta) . TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.6 kDa (315aa) confirmed by MALDI-TOF
Additionnal Information
GTF2E2, Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B, ATGP1547-10 µg, ATGP1547-20 µg, ATGP1547-50 µg, ATGP1547-100 µg, ATGP1547-250 µg, ATGP1547-500 µg, ATGP1547-1 mg, ATGP1547-10, ATGP1547-20, ATGP1547-50, ATGP1547-100, ATGP1547-250, ATGP1547-500, ATGP1547-1
References & Citations
Kang S.-W., et al. (2000) Genes Cells. 5:251-263; ; Liu L., et al. (2009) J. Biol. Chem. 284:5165-5174
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_002086
Uniprot Accession Number
P29084
Species
Human
AA Sequence
MDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK

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