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Recombinant E.coli mdh protein

CAT: 0112-ATGP1487-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1487-020Size:20 µg
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Description
Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction) . Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E. coli mdh protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.
Product Name Alternative
Malate dehydrogenase, ECK3225, JW3205
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 1000unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.
Molecular Weight
34.9 kDa (336aa) confirmed by MALDI-TOF
Additionnal Information
Mdh, malate dehydrogenase, ECK3225, JW3205, ATGP1487-10 µg, ATGP1487-20 µg, ATGP1487-50 µg, ATGP1487-100 µg, ATGP1487-250 µg, ATGP1487-500 µg, ATGP1487-1 mg, ATGP1487-10, ATGP1487-20, ATGP1487-50, ATGP1487-100, ATGP1487-250, ATGP1487-500, ATGP1487-1
References & Citations
Goward CR., et al. (1994) . Protein Sci. 3 (10) : 1883-8.; ; McAlister-Henn L., et al. (1988) . Trends Biochem. Sci. 13 (5) : 178-81.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_417703
Uniprot Accession Number
P61889
Species
E.coli
AA Sequence
MKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

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