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Recombinant human MANF protein

CAT: 0112-ATGP1465-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1465-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mesencephalic astrocyte-derived neurotrophic factor, also known as MANF, is a 20 kDa member of the ARMET family of proteins. MANF was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. Recombinant human MANF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Mesencephalic astrocyte-derived neurotrophic factor, ARMET, ARP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
20.8 kDa (183aa) confirmed by MALDI-TOF
Additionnal Information
MANF, Mesencephalic astrocyte-derived neurotrophic factor, ARMET, ARP, ATGP1465-10 µg, ATGP1465-20 µg, ATGP1465-50 µg, ATGP1465-100 µg, ATGP1465-250 µg, ATGP1465-500 µg, ATGP1465-1 mg, ATGP1465-10, ATGP1465-20, ATGP1465-50, ATGP1465-100, ATGP1465-250, ATGP1465-500, ATGP1465-1
References & Citations
Shridhar V., et al. (1996) Cancer Res. 56:5576.; ; Petrova P S., et al. (2003) J Mol Neurosci. 20:173.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Neuroscience
NCBI Accession Number
NP_006001
Uniprot Accession Number
P55145
Species
Human
AA Sequence
LRPGD CEVCISYLGR FYQDLKDRDV TFSPATIENE LIKFCREARG KENRLCYYIG ATDDAATKII NEVSKPLAHH IPVEKICEKL KKKDSQICEL KYDKQIDLST VDLKKLRVKE LKKILDDWGE TCKGCAEKSD YIRKINELMP KYAPKAASAR TDL

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