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Recombinant human DARS protein

CAT: 0112-ATGP1454-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1454-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DARS, also known as aspartyl-tRNA synthetase, catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Recombinant human DARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Aspartyl-tRNA synthetase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
59.2 kDa (521aa)
Additionnal Information
DARS, Aspartyl-tRNA synthetase, ATGP1454-10 µg, ATGP1454-20 µg, ATGP1454-50 µg, ATGP1454-100 µg, ATGP1454-250 µg, ATGP1454-500 µg, ATGP1454-1 mg, ATGP1454-10, ATGP1454-20, ATGP1454-50, ATGP1454-100, ATGP1454-250, ATGP1454-500, ATGP1454-1
References & Citations
Guzzo C.M., et al. (2008) Biochem. Biophys. Res. Commun. 365:718-723; ; Imami K., et al. (2001) Anal. Sci. 24:161-166;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001340
Uniprot Accession Number
P14868
Species
Human
AA Sequence
MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT P

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