Recombinant human DARS protein

• Description:

  DARS, also known as aspartyl-tRNA synthetase, catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Recombinant human DARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

• Product Name Alternative:

  Aspartyl-tRNA synthetase

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 85% by SDS-PAGE

• Molecular Weight:

  59.2 kDa (521aa)

• Additionnal Information:

  DARS, Aspartyl-tRNA synthetase, ATGP1454-10 µg, ATGP1454-20 µg, ATGP1454-50 µg, ATGP1454-100 µg, ATGP1454-250 µg, ATGP1454-500 µg, ATGP1454-1 mg, ATGP1454-10, ATGP1454-20, ATGP1454-50, ATGP1454-100, ATGP1454-250, ATGP1454-500, ATGP1454-1

• References & Citations:

  Guzzo C.M., et al. (2008) Biochem. Biophys. Res. Commun. 365:718-723; ; Imami K., et al. (2001) Anal. Sci. 24:161-166;

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

• Scientific Category:

  Epigenomics (Transcription & Translation)

• NCBI Accession Number:

  NP_001340

• Uniprot Accession Number:

  P14868

• Species:

  Human

• AA Sequence:

  MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT P