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Recombinant human GGH protein

CAT: 0112-ATGP1399-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1399-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Gamma-glutamyl hydrolase, also known as GGH, is a homodimeric protein that catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates and is a central enzyme in folyl and antifolyl poly-gamma-glutamate metabolism. GGH may play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Recombinant human GGH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Gamma-glutamyl hydrolase, GH
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
35.9 kDa (315aa) confirmed by MALDI-TOF
Additionnal Information
GGH, ATGP1399-10 µg, ATGP1399-20 µg, ATGP1399-50 µg, ATGP1399-100 µg, ATGP1399-250 µg, ATGP1399-500 µg, ATGP1399-1 mg, ATGP1399-10, ATGP1399-20, ATGP1399-50, ATGP1399-100, ATGP1399-250, ATGP1399-500, ATGP1399-1
References & Citations
Rhee M S., et al. (1998) Mol Pharmacol. 53:1040-1046.; ; Galivan J., et al. (1999) Semin Oncol. 26:33-37.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_003869.1
Uniprot Accession Number
Q92820
Species
Human
AA Sequence
RPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVP VRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFD DGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTAN FHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPN AVKTAFYLAE FFVNEARKNN HHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD

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