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Recombinant human PHOSPHO2 protein

CAT: 0112-ATGP1367-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1367-250Size:250 µg
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Description
Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloacid dehalogenase (HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly. Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
30.3 kDa (265aa) confirmed by MALDI-TOF
Additionnal Information
PHOSPHO2, Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2, ATGP1367-10 µg, ATGP1367-20 µg, ATGP1367-50 µg, ATGP1367-100 µg, ATGP1367-250 µg, ATGP1367-500 µg, ATGP1367-1 mg, ATGP1367-10, ATGP1367-20, ATGP1367-50, ATGP1367-100, ATGP1367-250, ATGP1367-500, ATGP1367-1
References & Citations
Roberts SJ., et al. (2005) Biochim Biophys Acta. 1752 (1) :73-82.; ; Stewart A J., et al. (2003) Protein Eng. 16 (12) :889-95.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001008489
Uniprot Accession Number
Q8TCD6
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKILLV FDFDNTIIDD NSDTWIVQCA PNKKLPIELR DSYRKGFWTE FMGRVFKYLG DKGVREHEMK RAVTSLPFTP GMVELFNFIR KNKDKFDCII ISDSNSVFID WVLEAASFHD IFDKVFTNPA AFNSNGHLTV ENYHTHSCNR CPKNLCKKVV LIEFVDKQLQ QGVNYTQIVY IGDGGNDVCP VTFLKNDDVA MPRKGYTLQK TLSRMSQNLE PMEYSVVVWS SGVDIISHLQ FLIKD

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