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Recombinant human MVD protein

CAT: 0112-ATGP1354-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1354-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MVD, also known as diphosphomevalonate decarboxylase, catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Recombinant human MVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Diphosphomevalonate decarboxylase, MPD
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
45.6 kDa (420aa) confirmed by MALDI-TOF
Additionnal Information
MVD, diphosphomevalonate decarboxylase, MPD, ATGP1354-10 µg, ATGP1354-20 µg, ATGP1354-50 µg, ATGP1354-100 µg, ATGP1354-250 µg, ATGP1354-500 µg, ATGP1354-1 mg, ATGP1354-10, ATGP1354-20, ATGP1354-50, ATGP1354-100, ATGP1354-250, ATGP1354-500, ATGP1354-1
References & Citations
Toth M.J., et al. (1996) J. Biol. Chem. 271:7895-7898; ; Molina H., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:2199-2204
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_002452
Uniprot Accession Number
P53602
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASEKPLAAV TCTAPVNIAV IKYWGKRDEE LVLPINSSLS VTLHQDQLKT TTTAVISKDF TEDRIWLNGR EEDVGQPRLQ ACLREIRCLA RKRRNSRDGD PLPSSLSCKV HVASVNNFPT AAGLASSAAG YACLAYTLAR VYGVESDLSE VARRGSGSAC RSLYGGFVEW QMGEQADGKD SIARQVAPES HWPELRVLIL VVSAEKKLTG STVGMRASVE TSPLLRFRAE SVVPARMAEM ARCIRERDFP SFAQLTMKDS NQFHATCLDT FPPISYLNAI SWRIIHLVHR FNAHHGDTKV AYTFDAGPNA VIFTLDDTVA EFVAAVWHGF PPGSNGDTFL KGLQVRPAPL SAELQAALAM EPTPGGVKYI IVTQVGPGPQ ILDDPCAHLL GPDGLPKPAA

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