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Recombinant human PGK2 protein

CAT: 0112-ATGP1349-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1349-250Size:250 µg
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Description
PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
46.9 kDa (437aa) confirmed by MALDI-TOF
Additionnal Information
PGK2, Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS, ATGP1349-10 µg, ATGP1349-20 µg, ATGP1349-50 µg, ATGP1349-100 µg, ATGP1349-250 µg, ATGP1349-500 µg, ATGP1349-1 mg, ATGP1349-10, ATGP1349-20, ATGP1349-50, ATGP1349-100, ATGP1349-250, ATGP1349-500, ATGP1349-1
References & Citations
Svaasand EK. et al. (2007) Muscle Nerve. 36:679-84.; ; Valentin C. et al. (1998) Hum Mutat. 12 (4) :280-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_620061
Uniprot Accession Number
P07205
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM

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