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Recombinant human TRPT1 protein

CAT: 0112-ATGP1327-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1327-250Size:250 µg
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Description
TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
TRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.3 kDa (277aa) confirmed by MALDI-TOF
Additionnal Information
TRPT1, tRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1, ATGP1327-10 µg, ATGP1327-20 µg, ATGP1327-50 µg, ATGP1327-100 µg, ATGP1327-250 µg, ATGP1327-500 µg, ATGP1327-1 mg, ATGP1327-10, ATGP1327-20, ATGP1327-50, ATGP1327-100, ATGP1327-250, ATGP1327-500, ATGP1327-1
References & Citations
Hu Q.-D., et al. (2003) Cell. Mol. Life Sci. 60:1725-1732; ; Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001153862
Uniprot Accession Number
Q86TN4
Species
Human
AA Sequence
MNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ

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