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Recombinant human PSMB2 protein

CAT: 0112-ATGP1321-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1321-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Proteasome subunit beta type-2, also known as PSMB2, is a member of the proteasome T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Recombinant human PSMB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Proteasome subunit beta type-2, HC7-I
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
24.9 kDa (221aa) confirmed by MALDI-TOF
Additionnal Information
PSMB2, Proteasome subunit beta type-2, HC7-I, ATGP1321-10 µg, ATGP1321-20 µg, ATGP1321-50 µg, ATGP1321-100 µg, ATGP1321-250 µg, ATGP1321-500 µg, ATGP1321-1 mg, ATGP1321-10, ATGP1321-20, ATGP1321-50, ATGP1321-100, ATGP1321-250, ATGP1321-500, ATGP1321-1
References & Citations
Morimoto Y., et al. (1995) J Biochem. 117:471-474.; ; Wnzel T., et al. (1995) Nat Struct Biol. 2:199-204.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002785
Uniprot Accession Number
P49721
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S

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