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Recombinant human NT5M protein

CAT: 0112-ATGP1164-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1164-020Size:20 µg
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Description
NT5M, also known as DNT2, belongs to the 5' (3') -deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2' (3') -phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome 17 p11. 2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unknown etiology. Recombinant human NT5M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
5' (3') -deoxyribonucleotidase mitochondrial, 5' (3') -deoxyribonucleotidase, mitochondrial, DNT2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.1 kDa (218aa) confirmed by MALDI-TOF
Additionnal Information
NT5M, 5' (3') -deoxyribonucleotidase mitochondrial, 5' (3') -deoxyribonucleotidase, mitochondrial, DNT2, ATGP1164-10 µg, ATGP1164-20 µg, ATGP1164-50 µg, ATGP1164-100 µg, ATGP1164-250 µg, ATGP1164-500 µg, ATGP1164-1 mg, ATGP1164-10, ATGP1164-20, ATGP1164-50, ATGP1164-100, ATGP1164-250, ATGP1164-500, ATGP1164-1
References & Citations
Rampazzo C., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:8239-8244; ; Rinaldo-Matthis A., et al. (2002) Nat. Struct. Biol. 9:779-787
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_064586.1
Uniprot Accession Number
Q9NPB1
Species
Human
AA Sequence
GGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC

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