Recombinant e.coli ppa protein

• Description:

  Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E. coli ppa protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative:

  Inorganic pyrophosphatase, ECK4222, JW4185

• Expression System:

  E.coli

• Antigen Species:

  E.coli

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 95% by SDS-PAGE

• Molecular Weight:

  21.9 kDa (196aa) confirmed by MALDI-TOF

• Additionnal Information:

  Ppa, inorganic pyrophosphatase, ECK4222, JW4185, ATGP1132-10 µg, ATGP1132-20 µg, ATGP1132-50 µg, ATGP1132-100 µg, ATGP1132-250 µg, ATGP1132-500 µg, ATGP1132-1 mg, ATGP1132-10, ATGP1132-20, ATGP1132-50, ATGP1132-100, ATGP1132-250, ATGP1132-500, ATGP1132-1

• References & Citations:

  Harold FM., et al (1966) Bacteriol Rev 30 (4) : 772-94.; ; Carman GM., et al (2006) Trends Biochem. Sci. 31 (12) : 694-9.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

• Scientific Category:

  Signal Transduction

• NCBI Accession Number:

  NP_418647

• Uniprot Accession Number:

  P0A7A9

• Species:

  E.coli

• AA Sequence:

  MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK