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Recombinant e.coli grxB protein

CAT: 0112-ATGP1040-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1040-500Size:500 µg
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Description
GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.5 kDa (235aa) confirmed by MALDI-TOF
Additionnal Information
GrxB, glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2, ATGP1040-10 µg, ATGP1040-20 µg, ATGP1040-50 µg, ATGP1040-100 µg, ATGP1040-250 µg, ATGP1040-500 µg, ATGP1040-1 mg, ATGP1040-10, ATGP1040-20, ATGP1040-50, ATGP1040-100, ATGP1040-250, ATGP1040-500, ATGP1040-1
References & Citations
Holmgren A, et al. (2004) Antioxid. Redox. Signal. 6 (1) : 63-74.; ; Lopez-Maury L, et al. (2009) J Bacteriol. 191 (11) :3534-43.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
NP_415582
Uniprot Accession Number
P0AC59
Species
E.coli
AA Sequence
MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI

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