Recombinant e.coli grxB protein

• Description:

  GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative:

  Glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2

• Expression System:

  E.coli

• Antigen Species:

  E.coli

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 95% by SDS-PAGE

• Molecular Weight:

  26.5 kDa (235aa) confirmed by MALDI-TOF

• Additionnal Information:

  GrxB, glutaredoxin 2 (Grx2), glutaredoxin 2, Grx2, bA101E13.1, CGI-133, GRLX2, ATGP1040-10 µg, ATGP1040-20 µg, ATGP1040-50 µg, ATGP1040-100 µg, ATGP1040-250 µg, ATGP1040-500 µg, ATGP1040-1 mg, ATGP1040-10, ATGP1040-20, ATGP1040-50, ATGP1040-100, ATGP1040-250, ATGP1040-500, ATGP1040-1

• References & Citations:

  Holmgren A, et al. (2004) Antioxid. Redox. Signal. 6 (1) : 63-74.; ; Lopez-Maury L, et al. (2009) J Bacteriol. 191 (11) :3534-43.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

• Scientific Category:

  Redox Proteins

• NCBI Accession Number:

  NP_415582

• Uniprot Accession Number:

  P0AC59

• Species:

  E.coli

• AA Sequence:

  MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI