Recombinant e.coli msrA protein

• Description :

  Peptide methionine sulfoxide reductase A, also known msrA, is an enzyme that catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. This protein could have an important function as a repair enzyme for proteins that have been inactivated by oxidation. Recombinant E. coli msrA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative :

  Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase

• Expression System :

  E.coli

• Antigen Species :

  E.coli

• Tag :

  His-Tag

• Applications :

  SDS-PAGE

• Concentration :

  0.5 mg/mL (determined by Bradford assay)

• Purity :

  > 90% by SDS-PAGE

• Molecular Weight :

  25.4 kDa (232aa) confirmed by MALDI-TOF

• Additionnal Information :

  MsrA, Peptide methionine sulfoxide reductase A, ECK4215, JW4178, pms, pmsR, peptide-methionine (S) -S-oxide reductase, ATGP1036-10 µg, ATGP1036-20 µg, ATGP1036-50 µg, ATGP1036-100 µg, ATGP1036-250 µg, ATGP1036-500 µg, ATGP1036-1 mg, ATGP1036-10, ATGP1036-20, ATGP1036-50, ATGP1036-100, ATGP1036-250, ATGP1036-500, ATGP1036-1

• References & Citations :

  Kuschel L., et al. (1999) FEBS Letts.1:17-21.; ; Gabbita S., et al. (1999) J Neurochem. 4:1660-1666.

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

• Scientific Category :

  Redox Proteins

• NCBI Accession Number :

  NP_418640

• Uniprot Accession Number :

  P0A744

• Species :

  E.coli

• AA Sequence :

  MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA