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Recombinant human NDE1 protein

CAT: 0112-ATGP1027-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1027-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NDE1 is a cytoplasmic protein belonging to the nudE protein family. Phosphorylated during mitosis, it is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Recombinant human NDE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NuDE, NuDE, nudE nuclear distribution gene E homolog 1 (A. nidulans)
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
39.9 kDa (355aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
NDE1, Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NuDE, NuDE, nudE nuclear distribution gene E homolog 1 (A. nidulans), ATGP1027-10 µg, ATGP1027-20 µg, ATGP1027-50 µg, ATGP1027-100 µg, ATGP1027-250 µg, ATGP1027-500 µg, ATGP1027-1 mg, ATGP1027-10, ATGP1027-20, ATGP1027-50, ATGP1027-100, ATGP1027-250, ATGP1027-500, ATGP1027-1
References & Citations
Yan X., et al. (2003) Mol Cell Biol. 23 (4) :1239-50. ; ; Kitagawa M., et al. (2000) FEBS Lett. 479 (1-2) :57-62.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001137451
Uniprot Accession Number
Q9NXR1
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC

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