Recombinant e.coli hchA protein

• Description:

  HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative:

  Hsp31 molecular chaperone, ECK1963, JW1950, yedu, yzzC

• Expression System:

  E.coli

• Antigen Species:

  E.coli

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Purity:

  > 95% by SDS-PAGE

• Molecular Weight:

  33.3 kDa (303aa) confirmed by MALDI-TOF

• Additionnal Information:

  HchA, Hsp31 molecular chaperone, ECK1963, JW1950, yedu, yzzC, ATGP1023-10 µg, ATGP1023-20 µg, ATGP1023-50 µg, ATGP1023-100 µg, ATGP1023-250 µg, ATGP1023-500 µg, ATGP1023-1 mg, ATGP1023-10, ATGP1023-20, ATGP1023-50, ATGP1023-100, ATGP1023-250, ATGP1023-500, ATGP1023-1

• References & Citations:

  Malki A. et al. (2003) Biochem. Biophys. Res. Commun. 301:430-436.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

• Scientific Category:

  Heat Shock Proteins

• NCBI Accession Number:

  NP_416476

• Uniprot Accession Number:

  P31658

• Species:

  E.coli

• AA Sequence:

  MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG