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Recombinant human CTDSP1 protein

CAT: 0112-ATGP1013-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1013-050Size:50 µg
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Description
Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recombinant human CTDSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1, Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, NLIIF, SCP1, NIF3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
31.2 kDa (280aa) confirmed by MALDI-TOF
Additionnal Information
CTDSP1, Carboxy-terminal domain RNA polymerase II polypeptide A phosphatase 1, Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, NLIIF, SCP1, NIF3, ATGP1013-10 µg, ATGP1013-20 µg, ATGP1013-50 µg, ATGP1013-100 µg, ATGP1013-250 µg, ATGP1013-500 µg, ATGP1013-1 mg, ATGP1013-10, ATGP1013-20, ATGP1013-50, ATGP1013-100, ATGP1013-250, ATGP1013-500, ATGP1013-1
References & Citations
Maiello B., et al. (2001) Front Biosci. 6: 1358-1368.; ; Palancade B., et al. (2004) J Mol Biol. 335:415-424.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_872580
Uniprot Accession Number
Q9GZU7
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS

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