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Recombinant human SPOP protein

CAT: 0112-ATGP1009-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1009-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SPOP (speckle-type POZ protein), also known as TEF2, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF homology) domain and one C-terminal BTB/POZ domain. This protein inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. Through an interaction with CuL-3, SPOP is involved in ubiquitinylation and protein degradation. Recombinant human SPOP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Speckle-type POZ protein, TEF2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
44.3 kDa (394aa) confirmed by MALDI-TOF
Additionnal Information
SPOP, Speckle-type POZ protein, TEF2, ATGP1009-10 µg, ATGP1009-20 µg, ATGP1009-50 µg, ATGP1009-100 µg, ATGP1009-250 µg, ATGP1009-500 µg, ATGP1009-1 mg, ATGP1009-10, ATGP1009-20, ATGP1009-50, ATGP1009-100, ATGP1009-250, ATGP1009-500, ATGP1009-1
References & Citations
Kwon J.E., et al. (2006) J. Biol. Chem. 281:12664-12672; ; Furukawa M., et al. (2003) Nat. Cell Biol. 5:1001-1007
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 0.2M NaCl, 2mM EDTA
Scientific Category
Ubiquitination
NCBI Accession Number
NP_001007231
Uniprot Accession Number
O43791
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ CPFLGPPRKR LKQS

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