Products for Research Use Only
RegisterLogin

Recombinant human PIN4 protein

CAT: 0112-ATGP0998-100Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGP0998-100Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Specifications
Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Product Name Alternative
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Molecular Weight
18.8 kDa (176aa) confirmed by MALDI-TOF
Additionnal Information
PIN4, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17, ATGP0998-10 µg, ATGP0998-20 µg, ATGP0998-50 µg, ATGP0998-100 µg, ATGP0998-250 µg, ATGP0998-500 µg, ATGP0998-1 mg, ATGP0998-10, ATGP0998-20, ATGP0998-50, ATGP0998-100, ATGP0998-250, ATGP0998-500, ATGP0998-1
References & Citations
Uchida T., et al. (1999) FEBS Lett. 446:278-282; ; Fujiyama-Nakamura S., et al. (2009) Mol. Cell. Proteomics. 8:1552-1565
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
AAH93700
Uniprot Accession Number
Q9Y237
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK

Related Products

CatalogName
ATGD0020-010PIN4 cDNA
PIN3001-500Recombinant human Pin1 protein
PIN3001-100Recombinant human Pin1 protein
PIN3001-020Recombinant human Pin1 protein
ATGP0744-100Recombinant human PIN/DYNLL1 protein
ATGP0744-500Recombinant human PIN/DYNLL1 protein
ATGP0744-020Recombinant human PIN/DYNLL1 protein
ATGP3566-004Recombinant mouse Pin1 protein
ATGP3566-100Recombinant mouse Pin1 protein
ATGP3566-020Recombinant mouse Pin1 protein

Alternative Products

CatalogName
PROTQ9Y237-01Human PIN4 Recombinant Protein
ARP3397-01Recombinant Human PIN4 Protein
RP042223PIN4 Recombinant Protein (Human)
OPCD06193-200UGPIN4 Recombinant Protein
MBS7613833-01Recombinant Human PIN4
6387-100Human Recombinant PIN4
TP720981PIN4 (NM_006223) Human Recombinant Protein
TMPJ-01396-01PIN4 Protein, Human, Recombinant (His)

Popular Products