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Recombinant human NECAP2 protein

CAT: 0112-ATGP0973-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0973-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s alpha-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis. Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
NECAP endocytosis associated 2, FLJ10420
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
30.5 kDa (283aa) confirmed by MALDI-TOF
Additionnal Information
NECAP2, NECAP endocytosis associated 2, FLJ10420, ATGP0973-10 µg, ATGP0973-20 µg, ATGP0973-50 µg, ATGP0973-100 µg, ATGP0973-250 µg, ATGP0973-500 µg, ATGP0973-1 mg, ATGP0973-10, ATGP0973-20, ATGP0973-50, ATGP0973-100, ATGP0973-250, ATGP0973-500, ATGP0973-1
References & Citations
Miller GJ., et al. (2003) Nat Struct Biol. 10 (8) :599-606. ; ; Mattera R., et al. (2004) J Biol Chem. 279 (9) :8018-28.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_060560
Uniprot Accession Number
Q9NVZ3
Species
Human
AA Sequence
MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF

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