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Recombinant human EF-1 delta/EEF1D protein

CAT: 0112-ATGP0955-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0955-050Size:50 µg
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Description
Eukaryotic translation elongation factor 1 delta, also known as EEF1D, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Overexpression of EEF1D is associated with oesophageal carcinoma and may adversely affect the outcome of medulloblastomas. Recombinant human EEF1D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Eukaryotic translation elongation factor 1 delta, EF-1D, EF1D, Elongation factor 1-delta, Antigen NY-CO-4
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.2 kDa (301aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
EEF1D, Eukaryotic translation elongation factor 1 delta, EF-1D, EF1D, ATGP0955-10 µg, ATGP0955-20 µg, ATGP0955-50 µg, ATGP0955-100 µg, ATGP0955-250 µg, ATGP0955-500 µg, ATGP0955-1 mg, ATGP0955-10, ATGP0955-20, ATGP0955-50, ATGP0955-100, ATGP0955-250, ATGP0955-500, ATGP0955-1
References & Citations
De Bortoli M., et al. (2006) BMC Cancer. 6:223.; ; Cans C., et al. (2003) Proc Natl Acad Sci. 100:13892-13897.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001951
Uniprot Accession Number
P29692
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I