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Recombinant human BCKDHA protein

CAT: 0112-ATGP0944-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0944-100Size:100 µg
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Description
BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3) . Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSu, MSuD1, OVD1A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
47.8 kDa (421aa) confirmed by MALDI-TOF
Additionnal Information
BCKDHA, 2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSu, MSuD1, OVD1A, ATGP0944-10 µg, ATGP0944-20 µg, ATGP0944-50 µg, ATGP0944-100 µg, ATGP0944-250 µg, ATGP0944-500 µg, ATGP0944-1 mg, ATGP0944-10, ATGP0944-20, ATGP0944-50, ATGP0944-100, ATGP0944-250, ATGP0944-500, ATGP0944-1
References & Citations
Nobukuni Y., et al. (1993) Biochim. Biophys. Acta 1225:64-70; ; Funchal, C., et al. (2005) Metab. Brain Dis. 20: 205-217
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000700.1
Uniprot Accession Number
P12694
Species
Human
AA Sequence
MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K

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