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Recombinant human ACO1 protein

CAT: 0112-ATGP0871-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0871-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACO1 (aconitate hydratase) plays a role as an iron sensor. This enzyme catalyzes the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Recombinant human ACO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Cytoplasmic aconitate hydratase, ACONS, IREB1, IREBP, IREBP1, IRP1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
100.8 kDa (912aa)
Additionnal Information
ACO1, Cytoplasmic aconitate hydratase, ACONS, IREB1, IREBP, IREBP1, IRP1, ATGP0871-10 µg, ATGP0871-20 µg, ATGP0871-50 µg, ATGP0871-100 µg, ATGP0871-250 µg, ATGP0871-500 µg, ATGP0871-1 mg, ATGP0871-10, ATGP0871-20, ATGP0871-50, ATGP0871-100, ATGP0871-250, ATGP0871-500, ATGP0871-1
References & Citations
Vashisht A.A., et al (2009) Science 326:718-721; ; Salahudeen A.A., et al (2009) Science 326:722-726;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_002188
Uniprot Accession Number
P21399
Species
Human
AA Sequence
MSNPFAH LAEPLDPVQP GKKFFNLNKL EDSRYGRLPF SIRVLLEAAI RNCDEFLVKK QDIENILHWN VTQHKNIEVP FKPARVILQD FTGVPAVVDF AAMRDAVKKL GGDPEKINPV CPADLVIDHS IQVDFNRRAD SLQKNQDLEF ERNRERFEFL KWGSQAFHNM RIIPPGSGII HQVNLEYLAR VVFDQDGYYY PDSLVGTDSH TTMIDGLGIL GWGVGGIEAE AVMLGQPISM VLPQVIGYRL MGKPHPLVTS TDIVLTITKH LRQVGVVGKF VEFFGPGVAQ LSIADRATIA NMCPEYGATA AFFPVDEVSI TYLVQTGRDE EKLKYIKKYL QAVGMFRDFN DPSQDPDFTQ VVELDLKTVV PCCSGPKRPQ DKVAVSDMKK DFESCLGAKQ GFKGFQVAPE HHNDHKTFIY DNTEFTLAHG SVVIAAITSC TNTSNPSVML GAGLLAKKAV DAGLNVMPYI KTSLSPGSGV VTYYLQESGV MPYLSQLGFD VVGYGCMTCI GNSGPLPEPV VEAITQGDLV AVGVLSGNRN FEGRVHPNTR ANYLASPPLV IAYAIAGTIR IDFEKEPLGV NAKGQQVFLK DIWPTRDEIQ AVERQYVIPG MFKEVYQKIE TVNESWNALA TPSDKLFFWN SKSTYIKSPP FFENLTLDLQ PPKSIVDAYV LLNLGDSVTT DHISPAGNIA RNSPAARYLT NRGLTPREFN SYGSRRGNDA VMARGTFANI RLLNRFLNKQ APQTIHLPSG EILDVFDAAE RYQQAGLPLI VLAGKEYGAG SSRDWAAKGP FLLGIKAVLA ESYERIHRSN LVGMGVIPLE YLPGENADAL GLTGQERYTI IIPENLKPQM KVQVKLDTGK TFQAVMRFDT DVELTYFLNG GILNYMIRKM AK

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