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Recombinant human BPHL protein

CAT: 0112-ATGP0870-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0870-020Size:20 µg
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Description
BPHL is a Serine hydrolase which Belongs to the AB hydrolase superfamily. This family catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. This protein is expressed at high levels in liver and kidney and at lower levels in heart, intestine and skeletal muscle. It plays a role in detoxification processes. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Recombinant human BPHL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Valacyclovir hydrolase, BPH-RP, MCNAA, VACVASE
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
31.1 kDa (275aa) confirmed by MALDI-TOF
Additionnal Information
BPHL, Valacyclovir hydrolase, BPH-RP, MCNAA, VACVASE, ATGP0870-10 µg, ATGP0870-20 µg, ATGP0870-50 µg, ATGP0870-100 µg, ATGP0870-250 µg, ATGP0870-500 µg, ATGP0870-1 mg, ATGP0870-10, ATGP0870-20, ATGP0870-50, ATGP0870-100, ATGP0870-250, ATGP0870-500, ATGP0870-1
References & Citations
Lai L., et al. (2008) J. Biol. Chem. 283:9318-9327.; ; Puente X.S., et al. (1995) J. Biol. Chem. 270:12926-12932;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_004323
Uniprot Accession Number
Q86WA6
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSVTSAKVAV NGVQLHYQQT GEGDHAVLLL PGMLGSGETD FGPQLKNLNK KLFTVVAWDP RGYGHSRPPD RDFPADFFER DAKDAVDLMK ALKFKKVSLL GWSDGGITAL IAAAKYPSYI HKMVIWGANA YVTDEDSMIY EGIRDVSKWS ERTRKPLEAL YGYDYFARTC EKWVDGIRQF KHLPDGNICR HLLPRVQCPA LIVHGEKDPL VPRFHADFIH KHVKGSRLHL MPEGKHNLHL RFADEFNKLA EDFLQ

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