Recombinant human NIT2 protein

CAT: 0112-ATGP0819-050Size: 50 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP0819-050Size:50 µg
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Description
Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein. Recombinant human NIT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Omega-amidase NIT2, Nitrilase homolog 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
33 kDa (299aa) confirmed by MALDI-TOF
Additionnal Information
NIT2, Omega-amidase NIT2, Nitrilase homolog 2, ATGP0819-10 µg, ATGP0819-20 µg, ATGP0819-50 µg, ATGP0819-100 µg, ATGP0819-250 µg, ATGP0819-500 µg, ATGP0819-1 mg, ATGP0819-10, ATGP0819-20, ATGP0819-50, ATGP0819-100, ATGP0819-250, ATGP0819-500, ATGP0819-1
References & Citations
Barqliw K T., et al. (2008) Biochemistry. 47 (51) :13514-23.; ; Camarqo A., et al. (2007) Plant Cell. 19 (11) :3491-503.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_064587
Uniprot Accession Number
Q9NQR4
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTSFRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP

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