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Recombinant human TPI1 protein

CAT: 0112-ATGP0763-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0763-500Size:500 µg
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Description
TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Triosephosphate isomerase, TPI, MGC88108, TIM, TPI, Triose-phosphate isomerase 1. Triosephosphate isomerase 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
28.8 kDa (269aa) confirmed by MALDI-TOF
Additionnal Information
TPI1, triosephosphate isomerase, TPI, MGC88108, TIM, TPI, Triose-phosphate isomerase 1. Triosephosphate isomerase 1, ATGP0763-10 µg, ATGP0763-20 µg, ATGP0763-50 µg, ATGP0763-100 µg, ATGP0763-250 µg, ATGP0763-500 µg, ATGP0763-1 mg, ATGP0763-10, ATGP0763-20, ATGP0763-50, ATGP0763-100, ATGP0763-250, ATGP0763-500, ATGP0763-1
References & Citations
Maquat L.E., et al. (1985) J. Biol. Chem. 260:3748-3753; ; Rodriguez-Almazan C, et al. (2008) J Biol Chem. 22; ;283 (34) :23254-63
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_000356
Uniprot Accession Number
P60174
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

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