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Recombinant human CuL1 protein

CAT: 0112-ATGP0753-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0753-050Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cullin1, also known CuL1, is a core component of multiple cullin-RING-based SCF (SKP1-CuL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, it serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cullin1 may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Recombinant human CuL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Cullin-1, CuL-1, Cullin-1, MGC149834, MGC149835
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
49.4 kDa (430aa) confirmed by MALDI-TOF
Additionnal Information
CuL1, Cullin-1, CuL-1, Cullin-1, MGC149834, MGC149835, ATGP0753-10 µg, ATGP0753-20 µg, ATGP0753-50 µg, ATGP0753-100 µg, ATGP0753-250 µg, ATGP0753-500 µg, ATGP0753-1 mg, ATGP0753-10, ATGP0753-20, ATGP0753-50, ATGP0753-100, ATGP0753-250, ATGP0753-500, ATGP0753-1
References & Citations
Michel JJ., et al. (1998) Cell Growth Differ. 9 (6) :435-49.; ; Kipreos ET., et al. (1996) Cell. 85 (6) :829-39.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_003583
Uniprot Accession Number
Q13616
Species
Human
AA Sequence
MSSTRSQNPH GLKQIGLDQI WDDLRAGIQQ VYTRQSMAKS RYMELYTHVY NYCTSVHQSN QARGAGVPPS KSKKGQTPGG AQFVGLELYK RLKEFLKNYL TNLLKDGEDL MDESVLKFYT QQWEDYRFSS KVLNGICAYL NRHWVRRECD EGRKGIYEIY SLALVTWRDC LFRPLNKQVT NAVLKLIEKE RNGETINTRL ISGVVQSYVE LGLNEDDAFA KGPTLTVYKE SFESQFLADT ERFYTRESTE FLQQNPVTEY MKKAEARLLE EQRRVQVYLH ESTQDELARK CEQVLIEKHL EIFHTEFQNL LDADKNEDLG RMYNLVSRIQ DGLGELKKLL ETHIHNQGLA AIEKCGEAAL NDPKMYVQTV LDVHKKYNAL VMSAFNNDAG FVAALDKACG RFINNNAVTK

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