Recombinant human PPCDC protein

CAT: 0112-ATGP0748-020Size: 20 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP0748-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B) . The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4'-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4'-phosphopantothenate into 4'-phosphopantetheine. Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
24.6 kDa (224aa) confirmed by MALDI-TOF
Additionnal Information
PPCDC, Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585, ATGP0748-10 µg, ATGP0748-20 µg, ATGP0748-50 µg, ATGP0748-100 µg, ATGP0748-250 µg, ATGP0748-500 µg, ATGP0748-1 mg, ATGP0748-10, ATGP0748-20, ATGP0748-50, ATGP0748-100, ATGP0748-250, ATGP0748-500, ATGP0748-1
References & Citations
Strauss E., et al. (2004) Biochemistry. 43 (49) :15520-33. ; ; Daugherty M., et al. (2002) J Biol Chem. 277 (24) :21431-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Metabolism
NCBI Accession Number
AAH14409
Uniprot Accession Number
Q96CD2
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS

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