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Recombinant human AA protein

CAT: 0112-ATGP0740-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0740-100Size:100 µg
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Description
AASDHPPT belongs to the P-Pant transferase superfamily. This protein catalyzes the post-translational modification of target proteins by phosphopantetheine and can transfer the 4-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN (in vitro) . It is detected in heart, skeletal muscle, placenta, testis, brain, pancreas, liver and kidney. Recombinant human AASDHPPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, CGI-80, LYS2, LYS5, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
36.4 kDa (316aa) confirmed by MALDI-TOF
Additionnal Information
AA, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, CGI-80, LYS2, LYS5, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, ATGP0740-10 µg, ATGP0740-20 µg, ATGP0740-50 µg, ATGP0740-100 µg, ATGP0740-250 µg, ATGP0740-500 µg, ATGP0740-1 mg, ATGP0740-10, ATGP0740-20, ATGP0740-50, ATGP0740-100, ATGP0740-250, ATGP0740-500, ATGP0740-1
References & Citations
Krupenko SA., et al. (2010) J Biol Chem. 285 (3) :1627-33.; ; Geraghty MT., et al. (2001) Mol Genet Metab. 72 (4) :336-42.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_056238
Uniprot Accession Number
Q9NRN7
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MEGVRWAFSC GTWLPSRAEW LLAVRSIQPE EKERIGQFVF ARDAKAAMAG RLMIRKLVAE KLNIPWNHIR LQRTAKGKPV LAKDSSNPYP NFNFNISHQG DYAVLAAEPE LQVGIDIMKT SFPGRGSIPE FFHIMKRKFT NKEWETIRSF KDEWTQLDMF YRNWALKESF IKAIGVGLGF ELQRLEFDLS PLNLDIGQVY KETRLFLDGE EEKEWAFEES KIDEHHFVAV ALRKPDGSRH QDVPSQDDSK PTQRQFTILN FNDLMSSAVP MTPEDPSFWD CFCFTEEIPI RNGTKS

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