Recombinant human HARS protein

CAT: 0112-ATGP0726-010Size: 10 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP0726-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Name Alternative
Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
59.8 kDa (532aa) confirmed by MALDI-TOF
Additionnal Information
HARS, Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic, ATGP0726-10 µg, ATGP0726-20 µg, ATGP0726-50 µg, ATGP0726-100 µg, ATGP0726-250 µg, ATGP0726-500 µg, ATGP0726-1 mg, ATGP0726-10, ATGP0726-20, ATGP0726-50, ATGP0726-100, ATGP0726-250, ATGP0726-500, ATGP0726-1
References & Citations
Raben N., et al. (1992) Nucleic Acids Res. 20 (5) :1075-81. ; ; O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294 (3) :609-14.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002100
Uniprot Accession Number
P12081
Species
Human
AA Sequence
MAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC

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