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Recombinant human DIRAS1 protein

CAT: 0112-ATGP0724-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0724-050Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DIRAS1 belongs to a distinct branch of the functionally diverse Ras superfamily of monomeric GTPases. Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. Recombinant human DIRAS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
GTP-binding protein Di-Ras1., Di-Ras1, FLJ42681, GBTS1, RIG, GTP-binding protein Di-Ras1.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
24.1 kDa (215aa) confirmed by MALDI-TOF
Additionnal Information
DIRAS1, GTP-binding protein Di-Ras1., Di-Ras1, FLJ42681, GBTS1, RIG, GTP-binding protein Di-Ras1., ATGP0724-10 µg, ATGP0724-20 µg, ATGP0724-50 µg, ATGP0724-100 µg, ATGP0724-250 µg, ATGP0724-500 µg, ATGP0724-1 mg, ATGP0724-10, ATGP0724-20, ATGP0724-50, ATGP0724-100, ATGP0724-250, ATGP0724-500, ATGP0724-1
References & Citations
Ellis CA., et al. (2002) Proc Natl Acad Sci u S A. 99 (15) :9876-81. ; ; Kontani K., et al. (2002) J Biol Chem. 277 (43) :41070-8.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 1mM EDTA
Scientific Category
Cancer
NCBI Accession Number
NP_660156.1
Uniprot Accession Number
O95057
Species
Human
AA Sequence
MPEQSNDYRV VVFGAGGVGK SSLVLRFVKG TFRDTYIPTI EDTYRQVISC DKSVCTLQIT DTTGSHQFPA MQRLSISKGH AFILVFSVTS KQSLEELGPI YKLIVQIKGS VEDIPVMLVG NKCDETQREV DTREAQAVAQ EWKCAFMETS AKMNYNVKEL FQELLTLETR RNMSLNIDGK RSGKQKRTDR VKGKC

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