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Recombinant mouse LYPLA1 protein

CAT: 0112-ATGP0720-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0720-500Size:500 µg
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Description
LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Name Alternative
Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.8 kDa (250aa) confirmed by MALDI-TOF
Additionnal Information
LYPLA1, Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1, ATGP0720-10 µg, ATGP0720-20 µg, ATGP0720-50 µg, ATGP0720-100 µg, ATGP0720-250 µg, ATGP0720-500 µg, ATGP0720-1 mg, ATGP0720-10, ATGP0720-20, ATGP0720-50, ATGP0720-100, ATGP0720-250, ATGP0720-500, ATGP0720-1
References & Citations
Schaloske R.H., et al. (2006) Biochim Biophys Acta. 1761:1246-1259.; ; Hiraoka M., et al. (2002) J. Biol. Chem 277:10090-10099.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_032892
Uniprot Accession Number
P97823
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID

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