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Recombinant human ECHS1 protein

CAT: 0112-ATGP0703-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0703-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Enoyl Coenzyme A hydratase short chain 1 mitochondrial, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.6 kDa (284aa) confirmed by MALDI-TOF
Additionnal Information
ECHS1, Enoyl Coenzyme A hydratase short chain 1 mitochondrial, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, ATGP0703-10 µg, ATGP0703-20 µg, ATGP0703-50 µg, ATGP0703-100 µg, ATGP0703-250 µg, ATGP0703-500 µg, ATGP0703-1 mg, ATGP0703-10, ATGP0703-20, ATGP0703-50, ATGP0703-100, ATGP0703-250, ATGP0703-500, ATGP0703-1
References & Citations
Kanazawa M., et al. (1993) Enzyme Protein. 47:9-13; ; Stern J R., et al. (1956) J Biol Chem. 218:985-1002.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
AAH08906
Uniprot Accession Number
P30084
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

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