Recombinant human CBFB protein

CAT: 0112-ATGP0667-050Size: 50 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP0667-050Size:50 µg
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Description
CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e. g., RuNX1) and osteogenesis (e. g., RuNX2) . This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit. Recombinant human CBFB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Core-binding factor beta subunit, PEBP2B (polyomavirus enhancer binding protein b), PEA2, CBF-beta, Core-binding factor, beta subunit
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
23.6 kDa (202aa) confirmed by MALDI-TOF
Additionnal Information
CBFB, Core-binding factor beta subunit, PEBP2B (polyomavirus enhancer binding protein b), PEA2, CBF-beta, Core-binding factor, beta subunit, ATGP0667-10 µg, ATGP0667-20 µg, ATGP0667-50 µg, ATGP0667-100 µg, ATGP0667-250 µg, ATGP0667-500 µg, ATGP0667-1 mg, ATGP0667-10, ATGP0667-20, ATGP0667-50, ATGP0667-100, ATGP0667-250, ATGP0667-500, ATGP0667-1
References & Citations
Speck NA., et al. (2009) Blood. 113 (13) :3070-9.; ; Ito Y., et al. (2008) Blood. 112 (9) :3777-87.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM MES buffer (pH 6.0) containing 0.1mM PMSF, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001746
Uniprot Accession Number
Q13951
Species
Human
AA Sequence
MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT REFEDRDRSH REEMEVRVSQ LLAVTGKKTT RP

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