Recombinant human CBFB protein

• Description:

  CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e. g., RuNX1) and osteogenesis (e. g., RuNX2) . This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit. Recombinant human CBFB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

• Product Name Alternative:

  Core-binding factor beta subunit, PEBP2B (polyomavirus enhancer binding protein b), PEA2, CBF-beta, Core-binding factor, beta subunit

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  0.5 mg/mL (determined by Bradford assay)

• Purity:

  > 85% by SDS-PAGE

• Molecular Weight:

  23.6 kDa (202aa) confirmed by MALDI-TOF

• Additionnal Information:

  CBFB, Core-binding factor beta subunit, PEBP2B (polyomavirus enhancer binding protein b), PEA2, CBF-beta, Core-binding factor, beta subunit, ATGP0667-10 µg, ATGP0667-20 µg, ATGP0667-50 µg, ATGP0667-100 µg, ATGP0667-250 µg, ATGP0667-500 µg, ATGP0667-1 mg, ATGP0667-10, ATGP0667-20, ATGP0667-50, ATGP0667-100, ATGP0667-250, ATGP0667-500, ATGP0667-1

• References & Citations:

  Speck NA., et al. (2009) Blood. 113 (13) :3070-9.; ; Ito Y., et al. (2008) Blood. 112 (9) :3777-87.;

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM MES buffer (pH 6.0) containing 0.1mM PMSF, 10% glycerol

• Scientific Category:

  Epigenomics (Transcription & Translation)

• NCBI Accession Number:

  NP_001746

• Uniprot Accession Number:

  Q13951

• Species:

  Human

• AA Sequence:

  MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT REFEDRDRSH REEMEVRVSQ LLAVTGKKTT RP