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Recombinant human EDF1 protein

CAT: 0112-ATGP0655-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0655-100Size:100 µg
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Description
Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. Recombinant human EDF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Endothelial differentiation-related factor 1, EDF-1, MBF1, Endothelial differentiation-related factor 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.4 kDa (156aa) confirmed by MALDI-TOF
Additionnal Information
EDF1, Endothelial differentiation-related factor 1, EDF-1, MBF1, Endothelial differentiation-related factor 1, ATGP0655-10 µg, ATGP0655-20 µg, ATGP0655-50 µg, ATGP0655-100 µg, ATGP0655-250 µg, ATGP0655-500 µg, ATGP0655-1 mg, ATGP0655-10, ATGP0655-20, ATGP0655-50, ATGP0655-100, ATGP0655-250, ATGP0655-500, ATGP0655-1
References & Citations
Mariotti M., et al. (2000) J Biol Chem. 275:24047-24051.; ; Dragoni I., et al. (1998) J Biol Chem. 273: 31119-31124. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003783
Uniprot Accession Number
O60869
Species
Human
AA Sequence
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH

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