Recombinant human WARS protein

CAT: 0112-ATGP0627-500Size: 500 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP0627-500Size:500 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
WARS, also known as tryptophanyl-tRNA synthetase, belongs to the class I tRNA synthetase family. Two forms of tryptophanyl tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. It also regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Recombinant human WARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Tryptophanyl-tRNA synthetase cytoplasmic, GAMMA-2, IFI53, IFP53, Tryptophanyl-tRNA synthetase, cytoplasmic, TrpRS, hWRS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
55.3 kDa (491aa) confirmed by MALDI-TOF
Additionnal Information
WARS, Tryptophanyl-tRNA synthetase cytoplasmic, GAMMA-2, IFI53, IFP53, Tryptophanyl-tRNA synthetase, cytoplasmic, TrpRS, hWRS, ATGP0627-10 µg, ATGP0627-20 µg, ATGP0627-50 µg, ATGP0627-100 µg, ATGP0627-250 µg, ATGP0627-500 µg, ATGP0627-1 mg, ATGP0627-10, ATGP0627-20, ATGP0627-50, ATGP0627-100, ATGP0627-250, ATGP0627-500, ATGP0627-1
References & Citations
Nagano K., et al. (2004) Oncogene. 23 (9) :1693-703.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Angiogenesis
NCBI Accession Number
NP_776049
Uniprot Accession Number
P23381
Species
Human
AA Sequence
MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q

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