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Recombinant human SORD protein

CAT: 0112-ATGP0574-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0574-020Size:20 µg
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Description
SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357 amino acid member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37C.
Product Name Alternative
Sorbitol dehydrogenase, Sorbitol dehydrogenase L iditol 2 dehydrogenase, SDH, SORD, SORD 1, SORD1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 20,000pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37C.
Molecular Weight
40.4 kDa (377aa) confirmed by MALDI-TOF
Additionnal Information
SORD, Sorbitol dehydrogenase, Sorbitol dehydrogenase L iditol 2 dehydrogenase, SDH, SORD, SORD 1, SORD1, ATGP0574-10 µg, ATGP0574-20 µg, ATGP0574-50 µg, ATGP0574-100 µg, ATGP0574-250 µg, ATGP0574-500 µg, ATGP0574-1 mg, ATGP0574-10, ATGP0574-20, ATGP0574-50, ATGP0574-100, ATGP0574-250, ATGP0574-500, ATGP0574-1
References & Citations
Baker MA., et al. (2010) Proteomics. 10 (3) :482-95. ; ; Sorger D., et al. (2009) Nucl Med Biol. 36 (1) :17-27.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Metabolism
NCBI Accession Number
AAH25295
Uniprot Accession Number
Q00796
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP

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