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Recombinant human SUB1 protein

CAT: 0112-ATGP0573-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0573-050Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SuB1, also known as PC4, is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SuB1 interacts with the activation domain of transcription factor IIA (TFIIA) and TATA-binding protein (TBP) -associated factors (TAFs) to directly bind to double stranded DNA. This protein induces both activation and repression of RNAPII basal transcription, depending on the presence or absence of these transcription factors and holoenzyme components. Recombinant human SuB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
SUB1 regulator of transcriptio, SUB1 homolog transcriptional regulator, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, PC4, SUB1 homolog, p14, PC4, RPO2TC1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16.5 kDa (147aa) confirmed by MALDI-TOF
Additionnal Information
SuB1, Activated RNA polymerase II transcriptional coactivator p15, p14, P15, PC4, Activated RNA polymerase II transcriptional coactivator p15 Activated RNA polymerase II transcriptional coactivator p15, Interferon related developmental regulator 1, MGC102747, PC4 LSB, Positive cofactor 4, RPO2TC1, SuB1, SuB1 homolog., ATGP0573-10 µg, ATGP0573-20 µg, ATGP0573-50 µg, ATGP0573-100 µg, ATGP0573-250 µg, ATGP0573-500 µg, ATGP0573-1 mg, ATGP0573-10, ATGP0573-20, ATGP0573-50, ATGP0573-100, ATGP0573-250, ATGP0573-500, ATGP0573-1
References & Citations
Tavenet A., et al. (2009) Proc Natl Acad Sci u S A. 106 (34) :14265-70. ; ; Scott KL., et al. (2009) Nature. 459 (7250) :1085-90.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006704.3
Uniprot Accession Number
P53999
Species
Human
AA Sequence
MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR KGISLNPEQW SQLKEQISDI DDAVRKL

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