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Recombinant human HAT1 protein

CAT: 0112-ATGP0507-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0507-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HAT1, also known as histone acetyltransferase 1, is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Recombinant HAT1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Histone acetyltransferase 1, KAT1, Histone acetyltransferase 1 HAT 1, Histidine aminotransferase 1, Histone acetyltransferase type B catalytic subunit
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
40 kDa (343aa) confirmed by MALDI-TOF
Additionnal Information
HAT1, Histone acetyltransferase 1, KAT1, Histone acetyltransferase 1 HAT 1, Histidine aminotransferase 1, Histone acetyltransferase type B catalytic subunit, ATGP0507-10 µg, ATGP0507-20 µg, ATGP0507-50 µg, ATGP0507-100 µg, ATGP0507-250 µg, ATGP0507-500 µg, ATGP0507-1 mg, ATGP0507-10, ATGP0507-20, ATGP0507-50, ATGP0507-100, ATGP0507-250, ATGP0507-500, ATGP0507-1
References & Citations
Verreault A., et al. (1998) Curr Biol. 8 (2) :96-108. ; ; Marmorstein R., et al. (2001) J Mol Biol. 311 (3) :433-44. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_003633
Uniprot Accession Number
O14929
Species
Human
AA Sequence
KKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD

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