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Recombinant human IVD protein

CAT: 0112-ATGP0487-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0487-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IVD (Isovaleryl Coenzyme A dehydrogenase) is a mitochondrial matrix enzyme that belongs to the acyl-CoA dehydrogenase family. IVD is a homotetrameric flavoenzyme which catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA. Defects of the IVD gene lead to ineffective isoforms that are the underlying cause of isovaleric acidemia. Recombinant human IVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Isovaleryl-CoA dehydrogenase mitochondrial, ACAD2, Isovaleryl-CoA dehydrogenase, mitochondrial Isovaleryl CoA dehydrogenase, Isovaleryl CoA dehydrogenase, mitochondrial, isovaleryl Coenzyme A dehydrogenase.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.3 kDa (415aa) confirmed by MALDI-TOF
Additionnal Information
IVD, ATGP0487-10 µg, ATGP0487-20 µg, ATGP0487-50 µg, ATGP0487-100 µg, ATGP0487-250 µg, ATGP0487-500 µg, ATGP0487-1 mg, ATGP0487-10, ATGP0487-20, ATGP0487-50, ATGP0487-100, ATGP0487-250, ATGP0487-500, ATGP0487-1
References & Citations
Hodges PW., et al. (2009) Eur J Neurosci. 29 (7) :1490-500. ; ; Rohini R., et al. (2009) Eur J Med Chem. 44 (8) :3330-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_002216.2
Uniprot Accession Number
P26440
Species
Human
AA Sequence
MHSLLPVDDA INGLSEEQRQ LRQTMAKFLQ EHLAPKAQEI DRSNEFKNLR EFWKQLGNLG VLGITAPVQY GGSGLGYLEH VLVMEEISRA SGAVGLSYGA HSNLCINQLV RNGNEAQKEK YLPKLISGEY IGALAMSEPN AGSDVVSMKL KAEKKGNHYI LNGNKFWITN GPDADVLIVY AKTDLAAVPA SRGITAFIVE KGMPGFSTSK KLDKLGMRGS NTCELIFEDC KIPAANILGH ENKGVYVLMS GLDLERLVLA GGPLGLMQAV LDHTIPYLHV REAFGQKIGH FQLMQGKMAD MYTRLMACRQ YVYNVAKACD EGHCTAKDCA GVILYSAECA TQVALDGIQC FGGNGYINDF PMGRFLRDAK LYEIGAGTSE VRRLVIGRAF NADFH

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