Recombinant human CDK2AP1 protein

• Description :

  CDK2AP1 is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. Also, interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes. Recombinant CDK2AP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative :

  Cyclin-dependent kinase 2 associated protein 1, CDKAP1, DOC1, CDKA1, Cyclin-dependent kinase 2 associated protein 1 CDK2 A1, CDK2 associated protein 1, CDK2AP1, Cyclin dependent kinase 2 associated protein 1 Deleted in oral cancer 1, DOC 1, DOC 1 related protein, DOC 1R, DOC1R, Putative oral cancer suppressor.

• Expression System :

  E.coli

• Antigen Species :

  Human

• Tag :

  His-Tag

• Applications :

  SDS-PAGE

• Concentration :

  0.25 mg/mL (determined by Bradford assay)

• Purity :

  > 95% by SDS-PAGE

• Molecular Weight :

  16.6 kDa (152aa) confirmed by MALDI-TOF

• Additionnal Information :

  CDK2AP1, Cyclin-dependent kinase 2 associated protein 1, CDKAP1, DOC1, CDKA1, Cyclin-dependent kinase 2 associated protein 1 CDK2 A1, CDK2 associated protein 1, CDK2AP1, Cyclin dependent kinase 2 associated protein 1 Deleted in oral cancer 1, DOC 1, DOC 1 related protein, DOC 1R, DOC1R, Putative oral cancer suppressor., ATGP0480-10 µg, ATGP0480-20 µg, ATGP0480-50 µg, ATGP0480-100 µg, ATGP0480-250 µg, ATGP0480-500 µg, ATGP0480-1 mg, ATGP0480-10, ATGP0480-20, ATGP0480-50, ATGP0480-100, ATGP0480-250, ATGP0480-500, ATGP0480-1

• References & Citations :

  Tsuji T., et al. (1998) J Biol Chem. 273 (12) :6704-9.; ; Hu MG., et al. (2004) Cancer Res. 64 (2) :490-9.

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 2mM DTT

• Scientific Category :

  Cell Cycle

• NCBI Accession Number :

  NP_004633

• Uniprot Accession Number :

  O14519

• Species :

  Human

• AA Sequence :

  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAAHMPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYTQGT GNSQVPQSKY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAETERNA RS