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Recombinant human HINT1 protein

CAT: 0112-ATGP0327-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0327-500Size:500 µg
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Description
HINT1, also known as Histidine triad nucleotide-binding protein 1, is a member of superfamily named for a near C-terminal HXHXHXX motif (H:Histidine, X:a hydrophobic amino acid) positioned at the alpha-phosphate of nucleotide substrates. This protein hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Although it was originally thought to be a protein kinase C inhibitor and act as a haplod-insufficient tumor suppressor including spontaneous tumor formation in Hint+/- and Hint-/-, its actual physiologic function is not known. Recombinant human HINT1 protein was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Histidine triad nucleotide-binding protein 1, Histidine triad nucleotide-binding protein 1, HINT, PKCI-1, PRKCNH1, Histidine triad nucleotide-binding protein 1 Adenosine 5' monophosphoramidase, HINT 1, Histidine triad nucleotide binding protein 1, PKCI 1, PKCI1, Protein kinase C inhibitor 1, Protein kinase C interacting protein 1.
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
13.8 kDa (126aa) confirmed by MALDI-TOF
Additionnal Information
HINT1, Histidine triad nucleotide-binding protein 1, Histidine triad nucleotide-binding protein 1, HINT, PKCI-1, PRKCNH1, Histidine triad nucleotide-binding protein 1 Adenosine 5' monophosphoramidase, HINT 1, Histidine triad nucleotide binding protein 1, PKCI 1, PKCI1, Protein kinase C inhibitor 1, Protein kinase C interacting protein 1., ATGP0327-10 µg, ATGP0327-20 µg, ATGP0327-50 µg, ATGP0327-100 µg, ATGP0327-250 µg, ATGP0327-500 µg, ATGP0327-1 mg, ATGP0327-10, ATGP0327-20, ATGP0327-50, ATGP0327-100, ATGP0327-250, ATGP0327-500, ATGP0327-1
References & Citations
Zhang YJ., et al. (2009), Cancer Lett, 275 (2) :277-84.; ; Pawel Bieganowski., et al. (2002) . J Biol Chem. 277 (13) :10852-10860;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005331
Uniprot Accession Number
P49773
Species
Human
AA Sequence
MADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG

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