NCALD cDNA

CAT: 0112-ATGD0224-010Size: 10 µgDry Ice: NoHazardous: No
CAT#:0112-ATGD0224-010Size:10 µg
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Description
NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
Chromosomal Location
8q22.2
Antigen Species
Human
Vector Type
PATGen (puc19-derived cloning vector)
Sequence
ATGGGGAAACAGAACAGCAAGCTGCGCCCGGAGGTCATGCAGGACTTGCTGGAAAGCACAGACTTTACAGAGCATGAGATCCAGGAATGGTATAAAGGCTTCTTGAGAGACTGCCCCAGTGGACATTTGTCAATGGAAGAGTTTAAGAAAATATATGGGAACTTTTTCCCTTATGGGGATGCTTCCAAATTTGCAGAGCATGTCTTCCGCACCTTCGATGCAAATGGAGATGGGACAATAGACTTTAGAGAATTCATCATCGCCTTGAGTGTAACTTCGAGGGGGAAGCTGGAGCAGAAGCTGAAATGGGCCTTCAGCATGTACGACCTGGACGGAAATGGCTATATCAGCAAGGCAGAGATGCTAGAGATCGTGCAGGCAATCTATAAGATGGTTTCCTCTGTAATGAAAATGCCTGAAGATGAGTCAACCCCAGAGAAAAGAACAGAAAAGATCTTCCGCCAGATGGACACCAATAGAGACGGAAAACTCTCCCTGGAAGAGTTCATCCGAGGAGCCAAAAGCGACCCGTCCATTGTGCGCCTCCTGCAGTGCGACCCGAGCAGTGCCGGCCAGTTCTGA
Additionnal Information
NCALD, NCALD, ATGD0224-10 µg, ATGD0224-20 µg, ATGD0224-50 µg, ATGD0224-100 µg, ATGD0224-250 µg, ATGD0224-500 µg, ATGD0224-1 mg, ATGD0224-10, ATGD0224-20, ATGD0224-50, ATGD0224-100, ATGD0224-250, ATGD0224-500, ATGD0224-1
Storage Conditions
Store the plasmid at -20C.
Formulation
Lyophilized
Scientific Category
Neuroscience
Omim
606722
NCBI Accession Number
NP_001035720.1
Species
Human
Preparation
1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.
RNA Reference
NM_001040630.1
DNA Size
582bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

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