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Human BHMT antibody

CAT: 0112-ATGA0584-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0584-100Size:100 µL
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Description
Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
Product Name Alternative
Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BHMT (1-406aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
3D6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, ATGA0584-10 µg, ATGA0584-20 µg, ATGA0584-50 µg, ATGA0584-100 µg, ATGA0584-250 µg, ATGA0584-500 µg, ATGA0584-1 mg, ATGA0584-010, ATGA0584-020, ATGA0584-050, ATGA0584-100, ATGA0584-250, ATGA0584-500, ATGA0584-01M
References & Citations
Mckeever MP et al., (1991) Clin Sci 81 (4) :551-556.; ; Norihiko F et al., (2001) Biochem J 129:313-320.; ; Francisco G et al., (2005) Biochem J 391:589-599.; ; Catherine M et al., (2005) Biochem J 392:443-448.; ; Janet D. S et al., (2006) Biochem J 395:363-371.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_001704
Uniprot Accession Number
Q93088
WB Description
The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Liver Tissue lysate
FACS Description
Flow cytometry analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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