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Human PP2C alpha/PPM1A antibody

CAT: 0112-ATGA0529-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0529-100Size:100 µL
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Description
Protein phosphatase 2C (PP2Calpha) is a Mn2+ - or Mg 2+ -dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNk MAPk pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signalingkinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Calpha, the prototypical member of the PPM family.
Product Name Alternative
Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PP2Calpha/PPM1A (1-382aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
P6C7
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2C-alpha, PP2Calpha, PP2CA, PP2C A, PP2C alpha, PDPC 1, PDP1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43, ATGA0529-10 µg, ATGA0529-20 µg, ATGA0529-50 µg, ATGA0529-100 µg, ATGA0529-250 µg, ATGA0529-500 µg, ATGA0529-1 mg, ATGA0529-010, ATGA0529-020, ATGA0529-050, ATGA0529-100, ATGA0529-250, ATGA0529-500, ATGA0529-01M
References & Citations
Lin X, et al., (2006) Cell. 125 (5) : 915-928. ; ; Duan X, et al., (2006) J Biol Chem. 281 (48) :36526-32.
Other References
Kim JS, et al. The interaction of hepatitis B virus X protein and protein phosphatase type 2 Calpha and its effect on IL-6. (Biochem Biophys Res Commun. 2006) {https://pubmed.ncbi.nlm.nih.gov/17055456/}; ; Kim YG, et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. (Arthritis Rheumatol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24980965/}; ; Pereira JM, et al. Infection Reveals a Modification of SIRT2 Critical for Chromatin Association. (Cell Rep. 2018) {https://pubmed.ncbi.nlm.nih.gov/29694890/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA , Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_066283
Uniprot Accession Number
P35813
WB Description
The cell lysates (40ug) and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha/PPM1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : Jurkat cell lysate; ; Lane 2 : HeLa cell lysate; ; Lane 3 : K-562 cell lysate; ; Lane 4 : MCF7 cell lysate; ; Lane 5 : A549 cell lysate; ; Lane 6 : Raji cell lysate; ; Lane 7 : Mouse kidney tissue lysate; ; Lane 8 : Mouse brain tissue lysate; ; Lane 9 : Mouse liver tissue lysate
IF Description
ICC/IF analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained with ATGA0529 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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