RegisterLogin

Human CD34 antibody

CAT: 112-ATGA0500-100Size: 100 µLDry Ice: NoHazardous: No
100 µLSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Hematopoietic progenitor cell antigen CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is likely an adhesion molecule with a role in early hematopoiesis by mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
Product Name Alternative
Hematopoietic progenitor cell antigen CD34, CD34 molecule
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CD34 (32-290aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT64F9
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CD34, ATGA0500-10 µg, ATGA0500-20 µg, ATGA0500-50 µg, ATGA0500-100 µg, ATGA0500-250 µg, ATGA0500-500 µg, ATGA0500-1 mg, ATGA0500-10, ATGA0500-20, ATGA0500-50, ATGA0500-100, ATGA0500-250, ATGA0500-500, ATGA0500-1
References & Citations
Satterthwaite A B., et al. (1992) Genomics. 12:788-794.; ; Krause D S., et al. (1994) Blood. 84:691-701.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_001020280
Uniprot Accession Number
P28906
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: TF1 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate
FACS Description
Flow cytometry analysis of CD34 in KG1 cells. The cell was stained with ATGA0500 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

Related Products

CatalogName
ATGP1964-050Recombinant human CD34 protein
ATGP1964-250Recombinant human CD34 protein
ATGP3447-250Recombinant human CD34 protein
ATGP1964-010Recombinant human CD34 protein
ATGP3447-050Recombinant human CD34 protein
ATGP3447-010Recombinant human CD34 protein
ATGA0331-050Human CD3 epsilon/CD3E antibody
ATGA0331-100Human CD3 epsilon/CD3E antibody
ATGP2000-020Recombinant human CD3 gamma/CD3G protein
ATGP3783-250Recombinant human CD3 delta/CD3D protein

Alternative Products

CatalogName
PT_74677-01CD34, Mouse Anti Human (CD34 Antibody)
E55A06114Human CD34 Antibody
PT_P10001-01CD34 Human Recombinant (CD34 Human)
ASB-G0600Human CD34 Antibody
E55A06113Human CD34 Antibody
V0023-01CD34 (ABT-CD34) Antibody
CO60411CD34, Human
ABP-PAB-00010CD34 antigen (CD34) polyclonal antibody
OM300573-01CD34 Human
117160Human CD34

Popular Products